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KMID : 0382419990250010037
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Korean Journal of Environmental Health Society
1999 Volume.25 No. 1 p.37 ~ p.43
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Inhibitory Effects of Chestnut Bark Extracts on Tyrosinase Activity and Melanin Biosynthesis
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Yang Min-Jin
Lim Se-Jin
Ann Hyoung-Soo
Kim Myoung-Ae
Ahn Hyung-Me
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Abstract
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The inhibitory effects of chestnut bark extracts (CIS, CIS-gel, CIS jelly) were investigated in vitro and in B-16 mouse melanoma cells on melanin biosynthesis which is closely related to hyperpigmentation. These extracts inhibited tyrosinase activity which converts dopa to dopachrome in the biosynthetic process and the extracts inhibited the production of melanin from dopachrome by auto-oxidation. B-16 cells treated by chestnut bark extracts showed that the viability was over 85% in MTT assay. Melanin production was inhibited significantly by chestnut bark extracts in vitro and in B-16 cells. Especially CIS showed 15 times stronger inhibition of melanin production than kojic aicd in vitro. CIS-gel and CIS showed much stronger inhibition of melanin production under concentration of 4 ㎍/㎖ than kojic acid in B-16 cells. These results suggest that the chestnut bark extracts can be used for a whitening agent of the skin.
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